The quest for old PC with ISA slot

While waiting for the new PCI 8255 interface board to reached Malaysia, we are still not finished yet with the old ISA 8255 Interface Board. Indeed, one of the possible reason why it is unable to read input data could be due to the faulty PC ISA slot itself.
In fact, it happened once three years ago where one of those 3 slots failed to communicate with the software. Once I moved the card into another slot, then its working fine.
However, for current case I had tried all of those 3 slots. Unfortunately, it still failed to read, but only only managed to write. Eventhough I believed that the most probable reason is the card itself is faulty due to mouse urine that causing the short circuit, since we have quite an ample time now, we just do it. Who knows if the lady luck is on our side?
After searching for old PC with ISA slot, out of a few PCs in the store, we found only one is available. The rest only got PCI slots and AGP slots. We assembled it and power it on. The power supply is OK and managed to turn ON the monitor as well. Unfortunately, the motherboard FAILED to turn ON. This means that for the time being we cannot test the card with other PC with ISA slots available. Until we found the new PC for further testing, then we can conclude the card is faulty.